Print this article
P. 30-31 /

AkzoNobel
Advantage of using chemically stable silica material in reversed phase chromatography at elevated pH

Keywords: ,

e-version image

Click HERE to download the PDF version

 

 



Reversed phase chromatography is the most common techniques for screening and purification of peptides and the mobile phase pH plays an important role in preparative purification of APIs. Traditional silica-based materials in reversed phase chromatography are however often limited to be operated in the pH range between 2 and 8, and exclude the possibilities to use high pH to achieve high purity and yield. A chemically stable organic/inorg