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TPB-technology A versatile tool for handling arginine-containing peptides in solution-phase peptide synthesis

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(2011

TPB-technology A versatile tool for handling arginine-containing peptides in solution-phase peptide synthesis,
Chemistry Today 
Vol. 29(4) July/August 2011
, 2-3

Arginine-rich peptides isan important category oftherapeutic peptides coveringdifferent indications such ascancer, anti-infectious diseasesas well as drug delivery systems(several cell penetratingpeptides contain arginines intheir sequences).The work-up after synthesisof peptides containingunprotected arginine residuescan be very troublesome dueto the low solubility in organicsolvents. However, theirtetraphenylborate (TPB) salt isinsoluble in water but soluble ina wide range of organic solvents, allowing an extractive and costeffective work-up (Scheme 1).TPB is introduced as sodium tetraphenylborate to an aqueoussolution of a water-soluble salt of an arginine-containing peptide.The formed TPB salt precipitates and can be recovered byfiltration or extraction. When recovered as solid, TPB