Jetting technology is a new patented method that produces agarose beads with a very narrow particle size distribution. Purolite is the first Agarose resin provider to produce process-scale volumes of a uniform particle size bead for the purification of recombinant protein and monoclonal antibodies.
Jetted resins demonstrate superior performance characteristics including:
- Enhanced pressure/flow properties
- Uniform particle sizes
- Increased dynamic binding capacities for high-productivity operations and easy scale-up
- Higher resolution/selectivity for demanding separations, combined with high yields
- Significantly more narrow particle size distribution. Jetted 35 µm particle size = 95% within 25-50 µm compared to a 35 µm batch emulsified = 95% within 25-100 µm (even after extensive sieving)
Jetted uniform beads also have several important benefits to the environment over batch emulsified resins. Jetting technology is a continuous manufacturing process that produces beads in an extremely more efficient way with very high yields. Since the beads are uniform in size, it removes the need for extensive sieving which generates considerably less waste. Another environmentally friendly advantage with this new technology is that it eliminates the need for high levels of organic solvents compared to batch emulsified resins.
Purolite Life Sciences has commercialized highly cross linked agarose, Praesto SP 35 µm (cation) and Praesto Q35 µm (anion) to provide high resolution polishing resins for recombinant proteins and other biomolecules.