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Determination of abamectin residues in fruit juice by fluorescence spectrum


*Corresponding author
1. Nanjing University of Aeronautics and Astronautics, College of Science, Nanjing, 210016, China
2. Huaiyin Institute of Technology, Huaian, 223003, China


In this work an analytical method for the determination of abamectin residues in fruit juices was developed based on fluorescence spectrum. The fluorescence spectra were obtained about the abamectin and the mixed system between abamectin and peach, apple juice using spectrofluorophotometer. The experimental results show that the effective characteristic fluorescence peak was found at 322nm and 334nm, respectively. Then the prediction models of avermectin residues were obtained in two kinds of fruit juices with good linearity and reproducibility. Moreover, the mean recoveries, relative standard deviation, limit of detection and limit of quantifications were obtained. According to the analysis results, the method using fluorescence spectrum is simple and screening for the rapid detection of abamectin pesticide residues in fruit juices.


Abamectin belongs to the family of avermectins which are macrocyclic lactones produced by the actinomycete streptomyces avemitilis (1, 2). It is a mixture of two components abamectin B1a (80%) and B1b (20%). Abamectin acts on insects by interfering with neural and neuromuscular transmission with the widely using (3). For example, abamectin is used to control insect and mite pests of a range of agronomic, fruit, vegetable and ornamental crops. However the standard of maximum residue level (MRL) about abamectin in vegetables and fruits is very strict all over the world (4), such as the MRL for abamectin in apple and peach is 0.02 in China (5).

The pesticide residues resulting from extensive use of abamectin in agricultural production must be dangerous for consumers. Especially it is used on apples and peaches, and its influence is more dangerous because these fruits are often used as the ingredients for baby foods. Therefore, it is of great significance to provide a suitable method for the determination of abamectin. Nowadays, there are many kinds of analysis methods about the pesticide residue detection, for example gas chroma ...