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Quality management in gene expression analysis

JASCHA SILBERMANN*, STEFAN WEINERT, MARCUS FROHME
*Corresponding author
Technical University of Applied Sciences Wildau, Bahnhofstraße 1, Wildau, 15745, Germany

Abstract

Necessary considerations for the establishmentof a quality-managed RT-PCR workflow for the analysis ofdifferential gene expression are introduced. Relevant qualitymanagement systems are considered and a basic workprogramme for the implementation of a laboratory qualitymanagement system in conformance with ISO 17025 isdiscussed. A general methodology for the implementation ofpreventive measures for laboratory processes is presented.


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