Advantage of using chemically stable silica material in reversed phase chromatography at elevated pH
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Reversed phase chromatography is the most common techniques for screening and purification of peptides and the mobile phase pH plays an important role in preparative purification of APIs. Traditional silica-based materials in reversed phase chromatography are however often limited to be operated in the pH range between 2 and 8, and exclude the possibilities to use high pH to achieve high purity and yield. A chemically stable organic/inorganic silica material is a good tool to have, in order to expand the pH working range for separations and purifications of peptides.
The consequence of working outside this range with regular silica is that the siloxane bond between the silane and the silica base material will break producing free silanes and exposed silanol groups at low pH or dissolution of the silica backbone at high pH. ...