Bioremediation techniques for naproxen and carbamazepine elimination.
Toxicity evaluation test

MARÍA DEL CARMEN MOLINA*, NATALIA GONZÁLEZ BENÍTEZ1, RAQUEL SIMARRO1, LUIS FERNANDO BAUTISTA2, CAROLINA VARGAS2, JESÚS PABLO GARCÍA CAMBERO3, EVA MARÍA DÍAZ1, MANUEL ARRAYÁS4, MARÍA ÁNGELES QUIJANO5
*Corresponding author
1. Departamento de Biología, Geología, Física y Química Inorgánica, ESCET, URJC. 28933 Móstoles, Madrid, Spain
2. Departamento de Tecnología Química y Energética, Tecnología Química y Ambiental, Tecnología Mecánica y Química Analítica. ESCET, URJC, 28933 Móstoles, Madrid, Spain
3. Área de Toxicología, Centro Nacional de Salud Ambiental, Instituto de la Salud Carlos III, 28220 Majadahonda, Madrid, Spain
4. Área de Electromagnetismo, URJC, 28943 Fuenlabrada, Madrid. Spain
5. Departamento de Ingeniería Civil: Hidráulica y Ordenación del Territorio, Escuela Técnica Superior de Ingeniería Civil, Universidad Politécnica de Madrid, 28014 Madrid, Spain

KEYWORDS: Naproxen, carbamazepine, wastewater treatment stations, toxicity.
ABSTRACT:
Isolation, identification and toxicological monitoring of new microbial consortia to degrade PPCP´s (pharmaceuticals and personal care products) have direct applications to improve the quality of effluents in WWTP (wastewater treatment plants). In this work, we present isolation and molecular identification of a microbial strain degrading frequently detected compounds in effluent water such as naproxen (analgesic) and carbamazepine (anticonvulsant), as well as detoxification evaluation by a zebrafish embryos biomodel. Naproxen was completely removed from real contaminated water samples within 7 days of culture by Serratia sp. However, the microbial strain was unable to remove carbamazepine remaining constant until the analysis finished. Toxicity test showed that naproxen elimination reduces mortality zebrafish embryos at 28%. As conclusion, Serratia sp. and possible other Enterobacteriaceae could be used in bioreactors and purifying plant biodiscs in order to achieve naproxen and probably others P ...
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