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Detergents in the purification, stabilization, and refolding of membrane proteins

Max-Planck-Institute for Developmental Biology Department I – Spemannstr. 35 – Tübingen, 72076, Germany


Detergents are widely used in biochemistry labs. Classical applicationsinclude the lysis of cells and the removal of proteins and lipids ( nucleic acid purification). In protein biochemistry, detergents aremostly used to solubilize and purify membrane proteins.Special applications include the stabilization and the refolding ofmembrane proteins from a denatured state. The purpose of this reviewis to display the potential of such detergent-based methods, usingrecent examples from life science literature.As detergents are not always compatible with standard laboratorymethods, common problems are discussed.


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