Modelling the inactivation
of PEF frequency and width
for soybean lipoxygenase in soymilk
Inactivation of pulsed electric fields (PEF) on soybean lipoxygenase (SLOX) was studied at different frequencyand width. Soymilk was exposed to pulse frequency from 100 to 600 Hz and pulse width from 1 to 5 μs width at strength 30kV/cm and treatment time 345 μs. The degree of inactivation of SLOX increased with the increase of frequency and width.About 87 percent activity of SLOX was inactivated when SLOX was exposed to PEF at 600 Hz and 5 μs width at 25°C,achieving maximum inactivation of SLOX. Inactivation of SLOX by PEF at different frequency and width was modelled. Thefirst-order kinetics exponential decay model described successfully the relation between the residual activity of SLOX andPEF parameters (frequency and width).